"They way they quantified the EBV, or EBNA-1, specific T-cells in this study was to stain them using so called HLA-class 1 pentamers (HLA-B35 & A02 restricted) loaded with know immunogenic peptides from EBNA-1 (HPVGEADYFEY & NLVPMVATV). The investigators did not assess whether, or not, these cells reacted, or proliferated, to these antigens. We know from many studies that T-cell can accommodate, or bind to, many different peptides that are loaded in so called HLA molecules, either on antigen presenting cells or loaded up into recombinant soluble HLA molecules (pentamers), however this does not tell us anything about whether they get turned on, or activated. For all we know these cells may be specific, or in love with, some unknown autoantigen, and just happen to bind to EBNA-1 peptide loaded HLA molecules. This study asks more questions than it answers. What we really need to know is a lot more about the function of these cells and whether or not they are reactive against EBV infected cells. More work needs to be done."
Erdura et al. EBNA1 antigen-specific CD8 + T cells in cerebrospinal fluid of patients with multiple sclerosis. Journal of Neuroimmunology. Available online 19 March 2016 - In Press.
Epidemiological data suggests that Epstein–Barr virus may be involved in the pathogenesis of Multiple Sclerosis (MS). We aimed to determine the frequency of CD8 +T cells specific for one EBNA1-derived epitope (HPVGEADYFEY) in cerebrospinal fluid (CSF) and blood of patients with MS and other inflammatory neurological diseases (OIND). The frequency of specific CD8 + T cells was assessed by HLA-class-I-binding pentamers restricted to HLA-B35. The frequency of HPVGEADYFEY-specific CD8 + T cells did neither differ significantly in blood nor CSF in MS compared to OIND, but was consistently higher in CSF compared to blood regardless of diagnosis.